Department of Biochemistry
1. ZEISS Axio Imager.Z2 Fluorescence Microscope
An upright fluorescence microscope with fully apochromatically corrected optics designed for detailed visualization and 3D documentation of cellular structures. The instrument is highly motorized, including the Z axis, objective turret, condenser, and filter modules, and is fully controlled via a touchscreen TFT display. The system includes a highly sensitive cooled monochrome CCD camera, advanced multichannel fluorescence software, and a PC workstation.
Contact person: prof. Jozef Nosek
2. QX 200 Digital PCR System
Complete laboratory platform designed for absolute quantification of target DNA or RNA molecules without the need for a standard curve. The setup consists of an automated droplet generator with HEPA filtration, a highly sensitive two-channel reader, and a control unit with advanced analytical software. It also includes a manual droplet generator and a 96-well thermal cycler.
Contact person: Dr. Stanislav Huszár
3. ÄKTA pure 25 M1 Protein Purification HPLC System
A chromatographic system for detection, separation, and purification of proteins ranging from micrograms to grams. The system operates at a flow rate of 0.001–25 ml/min at pressures up to 20 MPa, with automatic flow regulation to protect sample integrity. It includes modules for pH, conductivity, and simultaneous triple-wavelength UV detection. A flexible fraction collector for various tube and plate formats is included.
Contact person: Dr. Monika Záhorszká
4. Modular Oxygen Consumption Monitoring System Oroboros
An instrument designed for precise monitoring of oxygen consumption and key cellular or mitochondrial metabolites. The system is equipped with a highly stable Peltier thermostat, electromagnetically controlled stirrers, and an integrated suction system for easy removal of medium from the measurement chamber. Thanks to an advanced fluorescent sensor module and analytical software, it enables measurement of mitochondrial membrane potential, ATP production, and hydrogen peroxide formation.
Contact person: Dr. Ingrid Sveráková
5. MicroBeta 2 96-Well Scintillation Spectrophotometer
A specialized instrument for measuring radioactivity and luminescence in microplate samples, primarily used for monitoring incorporation of radioactive precursors into microorganisms and other cells. The system uses a unique detector with two automatically positioned photomultiplier tubes above and below the sample, enabling highly sensitive detection of ³H and ¹⁴C isotopes, including coincidence counting for increased sensitivity. The device features an automatic plate handler with capacity for up to 16 standard 96-well microplates.
Contact person: Dr. Júlia Zemanová
6. FilterMate Harvester 96-Well Plate Filtration and Washing System
An instrument designed for efficient vacuum-based harvesting and washing of samples from 96-well plates. The system consists of a 96-well head assembly, a vacuum pump, and a set of collection bottles with a tubing system.
Contact person: Dr. Júlia Zemanová
7. Dionex HPLC System with UV/VIS, RI, and RM Detectors
A high-performance liquid chromatography system designed for analytical and preparative separation as well as detection of radioactively labeled metabolites. The system combines a pump operating up to 60 MPa, a column oven for six columns, and an autosampler with a capacity of 100 vials. It is equipped with three detector types (UV-VIS, refractive index, and radiometric) and a high-capacity fraction collector.
Contact person: Dr. Adrian Pál
8. Typhoon IP Phosphorimager Scanner
An advanced laser-based imaging system for high-precision detection, acquisition, and analysis of radioactively labeled samples. The device offers a scanning area of at least 35 × 40 cm and very high resolution up to 10 µm with 16-bit image depth, enabling reliable work with a wide range of isotopes including ³H, ¹⁴C, and ³²P.
Contact person: Dr. Stanislav Huszár
9. Tri-Carb 4910 Liquid Scintillation Spectrometer
A highly sensitive instrument designed for accurate measurement of low-level radioactivity in samples. The system features a bidirectional automatic sample changer with a capacity of at least 400 large or 700 small vials, and advanced functions such as spectrum analysis, luminescence detection, and pulse shape discrimination.
Contact person: Dr. Dominik Juračka
10. High-Capacity Centrifuge Beckman Coulter Avanti J26 with Standard Rotor Set
High-capacity centrifuge designed for efficient separation of biological and chemical samples. It can process volumes up to 6 × 1000 ml at low to medium speeds.
Contact person: Dr. Martina Neboháčová
